Enzyme Catalase Labs

Varibles that affect Enzyme catalysis Reaction Rates Introduction Molecules be constantly moving in our bodies and in nature. When molecules move fast enough they collide into one another, lay offing chemical substance substance chemical receptions to occur. Factors such as temperature and concentrations rear either help increase or decrease these replys. (Jubenville. ) Enzymes are known as accelerator pedal because they are suitable to speed up reaction rates without creation destroyed or altered. They are able to encourage chemical reactions by decreasing the button of activation.The main function of enzyme catalase is to convert henry peroxide (H2O2) in our bodies into group O and water system. This can buoy be visually call forn when heat content peroxide is put on a wound and the peroxide bubbles. Enzymes can withal be effect in plant cells and fungi. (Huston. ) In this experiment we canvass the many variables that can change the rate of this reaction such as temperature, concentration levels of enzyme catalase and pH values. We are able to track these changes using an O2 Gas Sensor. (Enzymes. ) It is predicted that the rate of reaction will increase with temperature, pH levels and concentration. MethodsThree trial run underpasss were each alter with 5 mL of 3% henry peroxide and 5 mL of water. 10 drops of enzymes suspension was and then added to the Naigene domiciliate for each observation. attempt tubes one, devil and trine were added to the Naigene chamber respectively. The O2 Gas Sensor was laid on top of the Naigene chamber. The Naigene chamber was swirled for 60 seconds while the O2 Gas Sensor recorded the oxygen cosmos released during the reaction. The results were recorded. To study the effects of enzyme concentration on rate of reaction, four test tubes were each modify with 5 mL of 3% hydrogen peroxide and 5 mL of water.For each test observation 5, 10, 15 and 20 drops of enzyme catalase were primed(p) in the Naig ene chamber. The four test tubes were then added respectively. The Naigene chamber was swirled for 60 seconds while the O2 Gas Sensor recorded the oxygen world released during the reaction. To test the effect of temperature on reaction rate, three test tubes were each make full with 5 mL of 3% hydrogen peroxide and 5 mL of water. For each observation 10 drops of enzyme catalase was added to the Naigene chamber. tally tube one was placed in ice (temperature of 0-5 C). Test tube wo was placed in agency temperature (20-25 C). Test tube three was placed in warm water (30-35 C). Each test tube was held in this surroundings for five minutes. The Naigene chamber was swirled for 60 seconds while the O2 Gas Sensor recorded the oxygen be released during the reaction. To measure the effect of pH on catalase activity, three test tubes were each alter with 5 mL of 3% hydrogen peroxide and 5 mL of the appropriate pH buffer. Test tube one was filled with 5 mL of pH 4. Test tube two was fill ed with 5 mL of pH 7. Test tube three was filled with 5 mL of pH 10.Ten drops of enzyme catalase was added to the Naigene chamber and test tube one, two and three were added respectively. The O2 Gas Sensor was placed on top of the Naigene chamber and was swirled for 60 seconds. The O2 Gas Sensor then recorded the oxygen being released during the reaction. To measure the effect of different substrare concentrations on catalase reactions, three test tubes were utilise and labeled one, two and three. Test tube one was filled with 3 mL of 3% hydrogen peroxide and 7 mL of water. Test tube two was filled with 5 mL of 3% hydrogen peroxide and 5 mL of water.Test tube three was filled with 7 mL of 3% hydrogen peroxide and 3 mL of water. 10 drops of catalase suspension was placed in the Naigene bottle for each observation. Test tube one, two and three were then added to the Naigene chamber respectively. The O2 Gas Sensor was placed on top of the Naigene chamber and was swirled for 60 seconds . The O2 Gas Sensor then recorded the oxygen being released during the reaction. Results protrude 1 Test underground Number Rate of sign Reaction (m) 1 0. 085282 2 0. 074574 3 0. 09223 opine 1 The average reaction rate of the enzyme concentration. learn 2 Test Tube Drops of enzyme suspension Rate of Initial Reaction (m) 1 5 0. 060459 2 10 0. 071033 3 15 0. 0966 4 20 0. 15003 Figure 2 Changes in reaction rate due to the enzyme concentration. Figure 3 Test Tube Temperature measured Rate of Initial Reaction (m) 1 0-5 C 0. 038694 2 20-25 C 0. 084487 3 30-35 C 0. 065194 Figure 3 Changes in reaction rate due to the effects of different temperatures. Figure 4 Test Tube pH level Rate of Initial Reaction (m) 1 4 0. 013519 2 7 0. 045141 3 10 0. 049314Figure 4 Changes in reaction rate due to the pH level of the solution. Figure 5 Test Tube marrow of H2O2 Amount of H2O Rate of Initial Reaction (m) 1 3 7 0. 027672 2 5 5 0. 09168 3 7 3 0. 1087 Figure 5 Changes in reaction rate due to differe nt ratios of 3% hydrogen peroxide (H2O2) and water (H2O) In figure 1, we can see that the figures for each test were relatively the same. This is because the amount and type of chemicals employ in each test were the same. Figure two shows the initial rate of reaction increasing as the amount of enzyme suspension increases.This evidence demonstrates that the enzyme suspension allowed the reaction to occur more rapidly. Figure 3 demonstrates how temperature can play a role in rate of reaction. Our figures show that showed that rate of reaction was at a peak when in medium temperatures. Various levels of pH too played a role in rate of reaction. Figure 4 demonstrates that the higher(prenominal) the pH level, the faster reaction rate was. Figure 5 demonstrates that different ratios of H2O2 and H2O can alter the rate of reaction. The higher amounts of H2O2 allowed higher reaction rates then the lower concentrated amounts.Discussion Enzymes are prudent for almost all chemical reactions that take place. They are made up of proteins and are considered biocatalysts. (Jubenville. ) Biocatalysts can be described as when enzymes are used as catalysts to cause chemical reactions. (Novasep. ) Enzymes are known as catalyst because they are able to speed up reaction rates without being destroyed or altered. They are able to encourage chemical reactions by decreasing the energy of activation. (Huston. ) Enzymes attract substrates to their surface allowing chemical reactions to occur.Every enzyme haves reactive sites which allow very specific chemical reactions. The shape of the reactive site on the enzyme and the shape of the reactive site on the substrate must in all match in order for them to attract to one another. (Jubenville. ) Enzyme catalase can be found in various places of our bodies and nature. The main function of enzyme catalase is to convert hydrogen peroxide (H2O2) in our bodies into oxygen and water. This can be visually seen when hydrogen peroxide is put on a wound and the peroxide bubbles. (Huston. ) It can also be found in nature in plants and fungi.These molecules are constantly moving. When moving fast enough they collide into one another, allowing chemical reactions to occur. Factors such as temperature and concentrations can either help decrease or increase these reactions. Concentration of enzyme catalase for example, plays a huge role of how much oxygen will be broken down. Concentrations of enzyme catalase can also increase chances of a chemical reaction occurring because there are more molecules operable to do the job. The higher concentration of enzyme catalase used, the more oxygen will be released during reaction.The temperatures of the environment in which these reactions take place also play a polar role on the reaction. Heat for example, speeds up the movement of molecules allowing more of a chance for them to collide and cause a chemical reaction. (Jubenville. ) pH factors also change reaction rates. pH stands for p ower of hydrogen and measures the concentration on hydrogen ions in a solution. (Hyperphysics. ) The higher the concentration, the more hydrogen ions available to be broken down by enzymes. The more hydrogen or hydrogen eroxide in a solution, the more oxygen being released during the reaction. It was anticipate that reaction rates would increase with higher concentrations of H2O2, pH levels, temperatures and ratios. This was all turn out true through our observations of our experiment.Works Cited Biocatalysis Definition of Biocatalysis in Novasep Glossary. Biocatalysis Definition of Biocatalysis in Novasep Glossary. Novasep, 2010. Web. 1 Oct. 2012. &lthttp//www. novasep. com/misc/glossary. asp? defId=49&gt. (Novasep. ) Enzymes. Enzymes. Tuberose, n. d. Web. 27 Sept. 2012. &lthttp//www. uberose. com/Enzymes. hypertext mark-up language&gt. (Enzymes. ) Frequently Asked Questions A Learn A Houston Enzymes. Frequently Asked Questions A Learn A Houston Enzymes. Huston En zymes, 2010. Web. 1 Oct. 2012. &lthttp//www. houston-enzymes. com/learn/faq. php&gt. (Huston. ) Jubenville, Robert B. , and Richard G. Thomas. public Biology Laboratory Manual. Third ed. Dubuque Kendall/Hunt, 2008. Print. (Jubenville. ) PH. As a Measure of caustic and Base Properties. Hyperphysics, n. d. Web. 5 Oct. 2012. &lthttp//hyperphysics. phy-astr. gsu. edu/hbase/chemical/ph. html&gt. (Hyperphysics. )